Characterization of the Uncommon Lipid Families in Corynebacterium glutamicum by Mass Spectrometry
| Autor: | Raju V. V. Tatituri, Fong-Fu Hsu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification biology Corynebacterium Lipidome biology.organism_classification 01 natural sciences Mycolic acid Corynebacterium glutamicum Trehalose dimycolate 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry 010608 biotechnology Lipidomics lipids (amino acids peptides and proteins) Cytidine diphosphate Diacylglycerol kinase |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071614099 |
| DOI: | 10.1007/978-1-0716-1410-5_15 |
| Popis: | This book chapter provides readers the step-by-step instruction for cell growth, lipid isolation, and lipid analysis to obtain the lipidome of Corynebacterium glutamicum (C. glutamicum) in the genus Corynebacterium, a biotechnologically important bacterium. We separate the lipid families by preparative HPLC with an analytical C-8 column, followed by linear ion-trap multiple stage mass spectrometry (LIT MSn) with high-resolution mass measurement to define the structures of cytidine diphosphate diacylglycerol (CDP-DAG), glucuronosyl diacylglycerol (GlcA-DAG), α-D-mannopyranosyl-(1 → 4)-α-D-glucuronyl diacylglycerol (Man-GlcA-DAG), 1-mycolyl-2-acyl-phosphatidylglycerol (MA-PG), and acyl trehalose monomycolate (acyl-TMM) whose structures have been previously mis-assigned or not defined by mass spectrometric means. We also define the structures of mycolic acid, phosphatidylglycerol, phosphatidylinositol, cardiolipin, trehalose dimycolate lipids in the cell wall. The similarity of the lipidome to that in the Mycobacterium genera is consistent with the notion that Corynebacterium and Mycobacterium are gram-positive bacteria belonging to the suborder Corynebacterineae. |
| Databáze: | OpenAIRE |
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