Gain or loss of single chromosomes in wheat-rye addition lines and in 6x triticale detected by flow cytometry

Autor:	E. Heberle-Bors, J. Lafferty, M. Pfosser, A. Amon, Tamas Lelley
Rok vydání:	1995
Předmět:	Genetics medicine.diagnostic_test food and beverages Chromosome Aneuploidy Plant Science Triticale Biology medicine.disease Molecular biology Nuclear DNA Flow cytometry chemistry.chemical_compound chemistry embryonic structures medicine Poaceae Ploidy Agronomy and Crop Science DNA
Zdroj:	Plant Breeding. 114:555-557
ISSN:	1439-0523 0179-9541
DOI:	10.1111/j.1439-0523.1995.tb00858.x
Popis:	Flow cytometric DNA analysis was used to study changes in nuclear DNA content induced by the gain or loss of chromosomes or chromosome arms in wheat-rye addition lines and in triticale. The amount of DNA present was determined by comparison with internal reference values obtained from euploid plants. The smallest difference in the DNA level which could be detected between aneuploid and euploid plants was 1.84%. Thus, it was possible to determine aneuploidy in all cases, except when very short telocentric rye chromosomes were added to wheat (addition line 1RS). The study has proved that flow cytometry is an efficient and simple way to discriminate between euploid and aneuploid plants in wheat and triticale.
Databáze:	OpenAIRE
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