Attachment of CO dehydrogenase to the cytoplasmic membrane is limiting the respiratory rate ofPseudomonas carboxydovorans
| Autor: | Ortwin Meyer, Susanne Jacobitz, Frank Mayer, Manfred Rohde |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences 030306 microbiology Electron acceptor Microbiology Electron transport chain Cell membrane 03 medical and health sciences Electron transfer chemistry.chemical_compound Membrane Enzyme medicine.anatomical_structure chemistry Biochemistry Oxidoreductase Genetics medicine Molecular Biology Methylene blue 030304 developmental biology |
| Zdroj: | FEMS Microbiology Letters. 28:141-144 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1985.tb00780.x |
| Popis: | Cells of Pseudomonas carboxydovorans from the exponential growth phase revealed the major portion (87%) of CO dehydrogenase attached to the inner aspect of the cytoplasmic membrane. In stationary cells only about half of the total amount of the enzyme remained membrane-bound, and a drop of the CO-oxidizing activity with O2 was observed. The CO-oxidizing activity with the unphysiological electron acceptor methylene blue, which does not need any contact of the enzyme with the membrane, always exceeded that with O2. Measurements of respiration rates of extracts with different electron donors in addition to CO suggested that the electron transport chain is not rate-limiting. It is concluded that the electron flow from CO to O2 in intact cells of P. carboxydovorans is controlled by the amount of CO dehydrogenase attached to a membrane-bound electron acceptor. |
| Databáze: | OpenAIRE |
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