Expression of pMGN(-4k)LacZ-neo Gene Introduced into Embryonic Stem (ES) Cells in Chimeric Mouse Fetuses

Autor:	Kazuei Ito, Makoto Osonoi, Jutaro Takahashi
Rok vydání:	2001
Předmět:	Fetus Transgene Cell lac operon Cell Biology Transfection Biology Regenerative medicine Embryonic stem cell Molecular biology medicine.anatomical_structure Reproductive Medicine embryonic structures medicine Gene reproductive and urinary physiology
Zdroj:	Journal of Mammalian Ova Research. 18:123-126
ISSN:	1347-5878 1341-7738
DOI:	10.1274/jmor.18.123
Popis:	We attempted to produce chimeric mouse fetuses by using an embryonic stem (ES) cell subline, OM 3, which were transfected with pMGN(-4k)LacZ-neo transgene, and to analyze expression of the transgene in chimeric mouse fetuses. In embryonic days 11-13 (E11-13), no fetuses expressing the transgene were observed. In E14, three of 11 normal fetuses recovered showed the transgene expression. In these chimeric mouse fetuses, the regions expressing the transgene were found in a wide range of fetuses, including the temporal region of the head, trunk from neck to tail, and limbs. It was shown by histochemical analysis that these patterns of transgene expression were specific to skeletal muscles. These results indicated that OM 3 cells might be useful for analyses of mouse skeletal-myogenesis and for the development of basic techniques for skeletal muscles in regenerative medicine.
Databáze:	OpenAIRE
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