COVID-19 seroprevalence among healthcare workers of a large COVID Hospital in Rome reveals strengths and limits of two different serological tests
| Autor: | Patrizia Laurenti, Domenico Staiti, Domenico Pascucci, Marcello Di Pumpo, Francesco Castrini, E Carini, Brunella Posteraro, Michela Cicconi, Floriana D'Ambrosio, Maria Elena D'Alfonso, Massimo Fantoni, Daniele Ignazio La Milia, Francesco Maria De Simone, Rosalba Ricci, Federica Foti, Roberta Pastorino, Filippo Berloco, Fabio De-Giorgio, Stefania Boccia, Maurizio Sanguinetti, Paola Cattani, Andrea Cambieri, Umberto Moscato, Giuseppe Vetrugno, Maurizio Zega, Gennaro Capalbo |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
education.field_of_study Coronavirus disease 2019 (COVID-19) business.industry Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Population Gold standard (test) Venous blood Confidence interval Serology Internal medicine Medicine Seroprevalence business education |
| DOI: | 10.1101/2021.01.08.21249445 |
| Popis: | In several hospitals worldwide, healthcare workers are currently at the forefront against coronavirus disease 2019 (COVID-19). Since Fondazione Policlinico Universitario A. Gemelli (FPG) IRCCS has been enlisted as a COVID hospital, healthcare workers deployed to COVID wards were separated from those with limited or no exposure, whereas administrative staff was destined to work-from-home. Between June 4 and July 3 2020, an investigation was carried out to evaluate seroprevalence of SARS-CoV-2 IgG antibodies among employees of the FPG using point-of-care (POC) and venous blood tests. Sensitivity, specificity and predictive values were determined with reverse-transcription polymerase chain reaction (RT-PCR) on nasal/oropharyngeal swabs as gold standard.Four thousand, seven hundred seventy-seven participants were enrolled. Seroprevalence was 3.66% using the POC test and 1.19% using venous blood test, with a significant difference between the two (p < 0.05).POC sensitivity and specificity were, respectively, 63.64% (95% confidence interval (CI): 62.20% to 65.04%) and 96.64% (95% CI: 96.05% to 97.13%), while those of the venous blood test were, respectively, 78.79% (95% CI: 77.58% to 79.94%) and 99.36% (95% CI: 99.07% to 99.55%). Among low-risk population, point-of-care’s predictive values were 58.33% (positive) and 98.23% (negative) whereas venous blood test’s were 92.86% (positive) and 98.53% (negative). In conclusion, point-of-care tests have low diagnostic accuracy, while venous blood tests seem to show an overall poor reliability. |
| Databáze: | OpenAIRE |
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