Photocytotoxic effect of c60 fullerene against l1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation
| Autor: | Grynyuk, Franskevych Dv, Liudmyla Drobot, Svitlana Prylutska, Uwe Ritter, Ganna Pasichnyk, O. P. Matyshevska, Petukhov Dm |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
MAPK/ERK pathway
Cancer Research medicine.diagnostic_test 02 engineering and technology Cell cycle 021001 nanoscience & nanotechnology Nitric oxide Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Oncology chemistry Griess test Apoptosis medicine Biophysics Viability assay 0210 nano-technology Protein kinase A 030217 neurology & neurosurgery |
| Zdroj: | Experimental Oncology. 38:89-93 |
| ISSN: | 2312-8852 1812-9269 |
| DOI: | 10.31768/2312-8852.2016.38(2):89-93 |
| Popis: | Aim: To estimate the combined action of C60 fullerene and light irradiation on viability of L1210 leukemic cells, nitric oxide (NO) generation, p38 mitogen-activated protein kinase (MAPK) activity and cell cycle distribution. Methods: Cell viability was assessed by MTT test. Light-emitting diode lamp (λ = 410–700 nm, 2.45 J/cm2) was used for C60 fullerene photoexcitation. Nitrite level and NO-synthase activity were measured by Griess reaction and by conversion of L-arginine to L-citrulline, respectively. p38 MAPK activity was assessed by Western blot analysis. Cell cycle distribution was determined by flow cytometry. Results: It was shown that light irradiation of C60 fullerene-treated L1210 cells was accompanied by 55% decrease of their viability at 48 h of culture. Nitrite level measured as an index of reactive NO generation was increased at the early period after C60 fullerene photoexcitation due to activation of both constitutive and inducible NO-synthase isoforms. The simultaneous activation of p38 MAPK was detected. Accumulation of L1210 cells in sub-G1 phase of cell cycle was observed after C60 fullerene photoexcitation. Conclusion: Photoexcited C60 fullerene exerts cytotoxic effect, at least in part, through triggering production of reactive NO species and activation of p38 kinase apoptotic pathways in L1210 leukemic cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|