Single-step recovery and solid-phase refolding of inclusion body proteins using a polycationic purification tag

Autor:	Sophia Hober, Torbjörn Gräslund, My Hedhammar, Tove Alm
Rok vydání:	2006
Předmět:	Chromatography FLAG-tag Chemistry Phase (matter) Protein purification Ion chromatography Molecular Medicine One-Step Single step General Medicine Target protein Applied Microbiology and Biotechnology Inclusion bodies
Zdroj:	Biotechnology Journal. 1:187-196
ISSN:	1860-7314 1860-6768
DOI:	10.1002/biot.200500023
Popis:	A strategy for purification of inclusion body-forming proteins is described, in which the positively charged domain Z(basic) is used as a fusion partner for capture of denatured proteins on a cation exchange column. It is shown that the purification tag is selective under denaturing conditions. Furthermore, the new strategy for purification of proteins from inclusion bodies is compared with the commonly used method for purification of His(6)-tagged inclusion body proteins. Finally, the simple and effective means of target protein capture provided by the Z(basic) tag is further successfully explored for solid-phase refolding. This procedure has the inherited advantage of combining purification and refolding in one step and offers the advantage of eluting the concentrated product in a suitable buffer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::15782b4e1a0129babbe1e4fb29235460 https://doi.org/10.1002/biot.200500023 Zobrazit plný text záznamu Plný text