Comprehensive Multiphase NMR Spectroscopy of Intact 13C-Labeled Seeds
| Autor: | Michael Fey, Howard Hutchins, Henry J. Stronks, Leayen Lam, Heather L. Wheeler, Jochem Struppe, A. Gorissen, André J. Simpson, Myrna J. Simpson, Ronald Soong, Andre Sutrisno, Ries de Visser, Rajeev Kumar, Brian Andrew, Werner E. Maas, Martine Monette, Alan Hume, Malcolm M. Campbell, Sridevi Krishnamurthy |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Journal of Agricultural and Food Chemistry. 62:107-115 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf4045638 |
| Popis: | Seeds are complex entities composed of liquids, gels, and solids. NMR spectroscopy is a powerful tool for studying molecular structure but has evolved into two fields, solution and solid state. Comprehensive multiphase (CMP) NMR spectroscopy is capable of liquid-, gel-, and solid-state experiments for studying intact samples where all organic components are studied and differentiated in situ. Herein, intact 13C-labeled seeds were studied by a variety of 1D/2D 1H/13C experiments. In the mobile phase, an assortment of metabolites in a single 13C-labeled wheat seed were identified; the gel phase was dominated by triacylglycerides; the semisolid phase was composed largely of carbohydrate biopolymers, and the solid phase was greatly influenced by starchy endosperm signals. Subsequently, the seeds were compared and relative similarities and differences between seed types discussed. This study represents the first application of CMP-NMR to food chemistry and demonstrates its general utility and feasibility for s... |
| Databáze: | OpenAIRE |
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