The Epidermal Barrier to Schistosoma mansoni Infection*
| Autor: | William L. Epstein, Sinfu Tzeng, James H. McKerrow, Kimie Fukuyama |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Pathology medicine.medical_specialty integumentary system biology Epidermis (botany) Schistosomiasis biology.organism_classification medicine.disease Molecular biology law.invention chemistry Cytoplasm law parasitic diseases Keratin medicine Schistosoma mansoni Electron microscope Digestion Polyacrylamide gel electrophoresis |
| DOI: | 10.1159/000408674 |
| Popis: | The stratified epithelium serves as protection for underlying tissues and organs, and the structural fitness of the epidermal cells has been extensively reported. We studied the possible roles played by the epidermis as a barrier against the migration of Schistosoma mansoni larvae. Freshly shed cercariae were collected and placed on the back skin of 2-day-old rats. Electron microscopy of biopsies taken at various intervals showed the larvae and their secretory granules in keratinocytes in which the cytoplasm had become homogeneous in appearance. SDS gel electrophoresis showed the digestion of purified epidermal keratin (60K protein) by a proteinase secreted from cercariae, but this activity was inhibited by an inhibitor purified from epidermal cells. These findings suggest that epidermal cells function both structurally and chemically as a barrier against cercariae invasion. |
| Databáze: | OpenAIRE |
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