Immunohistochemical Detection of Newcastle Disease Virus in Chickens
| Autor: | Frederic J. Hoerr, Lockaby Sb, Yu Ms, Ellis Ac |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Pathology
medicine.medical_specialty animal structures Lung General Immunology and Microbiology Immunoperoxidase animal diseases Respiratory disease respiratory system Biology medicine.disease biology.organism_classification Newcastle disease Virology Staining Harderian gland medicine.anatomical_structure Food Animals embryonic structures medicine Respiratory epithelium Animal Science and Zoology Respiratory system |
| Zdroj: | Avian Diseases. 37:433 |
| ISSN: | 0005-2086 |
| DOI: | 10.2307/1591670 |
| Popis: | An immunoperoxidase histochemical technique utilizing a monoclonal primary antibody was developed for detection of Newcastle disease virus (NDV) antigen in tissues from chickens. The technique was applied to trachea, lung, spleen, Harderian gland, and cecal tonsil harvested from specific-pathogen-free (SPF) chickens at 2, 5, 7, 10, and 14 days postinoculation (PI) with NDV, and to corresponding tissues from commercial broiler chickens representing 30 cases of spontaneous respiratory disease. Positive staining occurred in the cytoplasm of respiratory epithelial cells in the trachea or bronchi of NDV-inoculated SPF chickens at 5 and 7 days PI. Staining also occurred in the respiratory epithelium of the trachea and bronchi of commercial broilers from seven of 30 cases of spontaneous respiratory disease. These results indicate that the immunoperoxidase technique has value as a rapid diagnostic test for Newcastle disease. |
| Databáze: | OpenAIRE |
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