The effect of chromophore charge on the incorporation efficiency of fluorescence-labeled nucleotides catalyzed by Taq DNA polymerase in matrix synthesis

Autor:	Yu. P. Lysov, V. E. Barsky, Alexander S. Zasedatelev, V. E. Kuznetsova, Sergey Surzhikov, Alexander V. Chudinov, V. E. Shershov, V. A. Vasiliskov, O. A. Zasedateleva, T. O. Guseinov, M. A. Spitsyn
Rok vydání:	2015
Předmět:	chemistry.chemical_classification biology Stereochemistry technology industry and agriculture Biophysics Chromophore Fluorescence Catalysis chemistry.chemical_compound chemistry Biochemistry biology.protein Nucleotide Matrix synthesis DNA Polymerase Taq DNA Polymerase
Zdroj:	Biophysics. 60:1013-1015
ISSN:	1555-6654 0006-3509
DOI:	10.1134/s0006350915060238
Popis:	The effect of the chromophore charge on the efficiency of incorporation of fluorescent-labeled nucleotides into DNA during PCR was studied using three dUTP derivatives that contain different fluorescent labels, i.e., electroneutral, positively charged, and negatively charged Cy5 analogs. dUTP labeled with an electroneutral Cy5 analog was shown to be most efficiently incorporated into DNA when Tag polymerase was used in PCR.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::13ca0d8da30343e95acdb3dc3748e020 https://doi.org/10.1134/s0006350915060238 Zobrazit plný text záznamu Full text from SpringerLink