Substrate–inhibitor specificity of cholinesterase and monoamine oxydase from optic ganglia of the pacific squid Todarodes pacificus and commander squid Berryteuthis magister
| Autor: | O. V. Yagodina, N. E. Basova, E. V. Rozengart, Vladimir S. Saakov |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Tryptamine Todarodes pacificus Squid Physiology Anatomy Tyramine Biology biology.organism_classification Biochemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Monoamine neurotransmitter chemistry Berryteuthis magister biology.animal biology.protein Serotonin 030217 neurology & neurosurgery Ecology Evolution Behavior and Systematics Cholinesterase |
| Zdroj: | Journal of Evolutionary Biochemistry and Physiology. 53:111-122 |
| ISSN: | 1608-3202 0022-0930 |
| Popis: | A comparative analysis of enzymological characteristics of cholinesterase (ChE) and monoamine oxydase (MAO) from the optic ganglia was performed in the Pacific squid Todarodes pacificus and Commander squid Berryteuthis magister caught in their four habitats across the Pacific Northwest. A substrate–inhibitor analysis revealed a homogeneity of T. pacificus and B. magister ChE preparations as well as homogeneity of T. pacificus vs. heterogeneity of B. magister MAO preparations. In case of thiocholine derivatives, the rate of hydrolysis induced by T. pacificus ChE was practically independent of the structure of the acyl group, whereas in case of B. magister ChE it was found to decrease in this substrate series. It is only T. pacificus MAO that was found to be able to deaminate also a diaminooxydase substrate histamine. ChE activity was higher in T. pacificus than in B. magister for the whole substrate series, while for MAO the same activity pattern was observed for tyramine, tryptamine and serotonin. In both squids, the sensitivity of ChE to organophosphorus inhibitors containing the dimethylbutyl group was by several orders of magnitude higher than that in mammals. The sensitivity of ChE to the siloxane reversible inhibitors was lower in T. pacificus ChE and much lower in B. magister than in mammals. |
| Databáze: | OpenAIRE |
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