Incidence of the potato late blight pathogen, Phytophthora infestans, in Syria and its mating type
Autor: | Walid Naffaa, A. Al-Daoude, Taissir Abou Al-Fadil, Soulima Ibrahim |
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Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
0301 basic medicine Mating type Veterinary medicine Incidence (epidemiology) fungi Pcr assay food and beverages Plant Science Horticulture Biology biology.organism_classification 01 natural sciences 03 medical and health sciences 030104 developmental biology Agronomy Specific primers Phytophthora infestans Oospore Blight Agronomy and Crop Science Pathogen 010606 plant biology & botany |
Zdroj: | Journal of Plant Diseases and Protection. 124:533-537 |
ISSN: | 1861-3837 1861-3829 |
DOI: | 10.1007/s41348-017-0130-8 |
Popis: | Late blight, caused by Phytophthora infestans (Mont.) de Bary, is a devastating disease found worldwide. During 2011, 2013 and 2014 cropping seasons, prevalence of potato late blight in all survived areas throughout the interior of Syria was examined and the disease incidence varied and ranged from 0 to 85.71%. Potato leave samples showing late blight symptoms were collected from five different regions known to grow potato intensively. Based on their morphological characteristics, 22 isolates were distinguished as P. infestans. Using two fungal specific primers, ITS3 and PINF2 in a PCR assay, all isolates were confirmed as P. infestans, while they produce ~ 450 bp band. Similarly, all confirmed P. infestans isolates were able to produce a 170-bp band when INF-1 and INF-2 primers were deployed indicating that studied isolates had merely mating type A1 and no isolate with mating type A2 was found which might explain the absence of oospores in considered areas. |
Databáze: | OpenAIRE |
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