Biosynthesis of porphyrins and corrins. 1. Proton and carbon-13 NMR spectra of (hydroxymethyl)bilane and uroporphyrinogens I and III

Autor:	I. Ichinose, R. C. Davies, N. E. Mackenzie, A. S. F. Boyd, A. I. Scott, J. N. S. Evans, R. L. Baxter
Rok vydání:	1986
Předmět:	Uroporphyrinogens biology Chemistry Stereochemistry Uroporphyrinogen III synthase Corrin Nuclear magnetic resonance spectroscopy Biochemistry Porphyrin chemistry.chemical_compound Porphobilinogen Uroporphyrinogen III biology.protein Organic chemistry Hydroxymethyl
Zdroj:	Biochemistry. 25:896-904
ISSN:	1520-4995 0006-2960
DOI:	10.1021/bi00352a024
Popis:	High-field NMR spectroscopic methods have been applied to study the reactions catalyzed by porphobilinogen (PBG) deaminase and uroporphyrinogen III (uro'gen III) cosynthase, which are the enzymes responsible for the formation of the porphyrin macrocycle. The action of these enzymes in the conversion of PBG, [2,11-13C]PBG, and [3,5-13C]PBG to uro'gens I and III has been followed by 1H and 13C NMR, and assignments are presented. The principal intermediate that accumulated was the correspondingly labeled (hydroxymethyl)bilane (HMB), the assignments for which are also presented.
Databáze:	OpenAIRE
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