Screening and simultaneous quantitative measurement of six sulfonylureas in serum by liquid chromatography/mass spectrometry with atmospheric-pressure chemical-ionization (APCI LC/MS)
| Autor: | F. Susanto, H. Reinauer |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
endocrine system
Chromatography Chemistry nutritional and metabolic diseases Atmospheric-pressure chemical ionization Mass spectrometry Biochemistry High-performance liquid chromatography Glibornuride Glibenclamide chemistry.chemical_compound Liquid chromatography–mass spectrometry medicine Gliquidone medicine.drug Glipizide |
| Zdroj: | Fresenius' Journal of Analytical Chemistry. 357:1202-1205 |
| ISSN: | 1432-1130 0937-0633 |
| DOI: | 10.1007/s002160050331 |
| Popis: | A method is described for the separation, identification and quantification of six sulfonylureas in serum by liquid chromatography/mass spectrometry with atmospheric-pressure chemical-ionization (APCI LC/MS). The sample pretreatment is based on single-step liquid-liquid extraction, using the 4-methylcyclohexyl analogue of glibenclamide as internal standard. Carbutamide and tolbutamide have been chosen as representative sulfonylureas of the first generation and glipizide, glibornuride, glibenclamide and gliquidone as those of the second generation, widely used in the treatment of diabetes mellitus. This method allows a screening of these sulfonylureas in serum and subsequently the quantification of the serum level in one run of measurement. Accuracy, precision, specificity and sensitivity for each sulfonylurea are presented and discussed. |
| Databáze: | OpenAIRE |
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