Production and characterisation of transgenic cauliflower plants containing abnormal chloroplasts

Autor:	Veera R.N. Chikkala, David M. Stalker, Gregory D. Nugent, Trevor W. Stevenson
Rok vydání:	2013
Předmět:	medicine.diagnostic_test Transgene fungi food and beverages Horticulture Biology Protoplast Phenotype Cell biology Chloroplast Transformation (genetics) Western blot Botany medicine Plastid Gene
Zdroj:	Scientia Horticulturae. 164:409-413
ISSN:	0304-4238
DOI:	10.1016/j.scienta.2013.09.041
Popis:	Macrochloroplasts, which can be produced by the imbalance expression of plastid division genes, may act as an alternative target tissue for the transformation of foreign genes especially by particle bombardment. This paper reports the production of stable transgenic cauliflower plants expressing cauliflower plastid division gene, BoMinD by using PEG mediated transformation of mesophyll protoplasts. Stable transgenic BoMinD cauliflower plants had abnormally shaped chloroplasts but these did not exhibit a true macrochloroplast or minichloroplast phenotype. Transgene number did not relate to the expression of the BoMinD transgene. Detection of total BoMinD by western blot suggests that the slight increase in total BoMinD levels resulted in honey-comb or doughnut shaped chloroplasts and irregular surface membrane chloroplasts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::102b0a9e79e951a5e7c95238d2021b1c https://doi.org/10.1016/j.scienta.2013.09.041 Zobrazit plný text záznamu Full Text from ScienceDirect