Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells
| Autor: | Koh-ei Toyoshima, Takashi Tsuji, Kyosuke Asakawa |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
integumentary system Regeneration (biology) Mesenchymal stem cell Organogenesis Ectoderm Biology Hair follicle Embryonic stem cell Cell biology Transplantation 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure otorhinolaryngologic diseases medicine sense organs Stem cell 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493969470 |
| DOI: | 10.1007/978-1-4939-6949-4_9 |
| Popis: | Hair follicles develop from the ectoderm in embryos and cyclically regenerate using proper spatiotemporal signaling molecules, which are conserved in organogenesis during adulthood. Previously, we demonstrated that bioengineered hair follicle germs could regenerate functional hair follicles via a three-dimensional cell manipulation technique, which we named the "organ germ method ." We could also regulate the type of hair follicle and pigmentation with correct structures by rearranging the source of the cells. In this article, we describe a detailed protocol for the regeneration of functional hair follicles and their stem cell niches by the rearrangement of embryonic or adult hair follicle-derived epithelial and mesenchymal cells. |
| Databáze: | OpenAIRE |
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