Oral Fibrosarcoma in Dog: Cytological, Histopathological and Immunohistochemical Diagnosis

Autor: Amishi ., Yamini Verma, Madhu Swamy, Shobha Jawre, Amita Dubey, Nidhi Rajput
Rok vydání: 2022
Předmět:
Zdroj: Indian Journal of Animal Research.
ISSN: 0976-0555
0367-6722
DOI: 10.18805/ijar.b-4873
Popis: Background: Canine oral fibrosarcomas are locally invasive malignant mesenchymal tumors and the third most frequently occurring neoplasia of oral cavity of dogs. The present study was conducted to record pathological features of oral fibrosarcomas in canines. Methods: During the study period (September 2020- April 2021), a total of 18 cases of canine oral neoplasia were reported and amongst these fibrosarcoma was diagnosed in five animals (27.78%). The blood samples were collected from all the dogs and the excised tumor mass was processed at Department of Veterinary Pathology, Co.V.Sc. and A.H., Jabalpur. Result: The hematological profile of all the five dogs showed no variation however; serum biochemical analysis revealed hypercalcemia. The serum MDA concentration was elevated in dogs with fibrosarcoma, indicating oxidative stress. The gross morphology of the tumors included red to pink colored irregular masses, hard in consistency and firmly attached to the underlying tissue. Cytologically, the impression smears revealed spindle shaped cells with round to elongated nuclei along with strands of collagen fibres. Upon histopathological examination proliferation of fibrous connective tissue was observed in the sub-mucosa. Higher magnification revealed several immature spindle shaped fibroblasts along with scanty amounts of collagenous matrix. Masson’s trichome staining also exhibited blue stained collagen deposition. Mitotic figures were numerous (8.4 mitosis/10 hpf). The tissue sections stained by modified silver colloid staining were examined at 1000 x magnification. Mean Ag-NOR count of the silver nitrate stained tissue sections was enumerated to be 9.15 dots/nucleus. Immunohistochemical examination detected CD31 immunopositivity in the tissue sections as brown staining of the infiltrating blood vessels, indicating neovascularization of the tumor.
Databáze: OpenAIRE
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