Detection and Identification of Human Type A Influenza Viruses by The Reverse Transcriptase-Polymerase Chain Reaction in Bulgaria

Autor:	T. Hadzhiolova, Rositsa Kotseva, S. Pavlova, Zlatko Kalvatchev
Rok vydání:	2006
Předmět:	biology Biochemistry (medical) Clinical Biochemistry Orthomyxoviridae virus diseases Hemagglutinin (influenza) Outbreak biology.organism_classification Virology Reverse transcriptase Subtyping Microbiology law.invention law biology.protein Typing Neuraminidase Polymerase chain reaction
Zdroj:	Laboratory Medicine. 37:161-165
ISSN:	1943-7730 0007-5027
Popis:	Introduction/Purpose: Reverse transcriptasepolymerase chain reaction (RT-PCR) for detection, typing, and subtyping of the hemagglutinin and neuraminidase genes of influenza type A (H3N2 and H1N1) viruses in patients' specimens has been developed and applied for the purpose of influenza virological surveillance in the 2003/2004 and 2004/2005 winter seasons. The technique was performed prior to the viral isolation, in order to determine the influenza outbreaks' etiological agent. Methods: During the 2003/2004 season, RTPCR was performed following an initial screening of 50 patients' samples by 3 other methods. In the 2004/2005 season, the method was used for direct identification of 77 clinical samples. Results/Discussion: All the samples tested were taken during the peak of influenza outbreaks. The report presented is the first one for the country to demonstrate an RT-PCR effective application in influenza virus detection in clinical samples from epidemic outbreaks.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0f42766f25c2b21750f6b1e6b0e8edb9 https://doi.org/10.1309/ugj4b6m6yq0wugt8 Zobrazit plný text záznamu