Granulocyte-macrophage colony-stimulating factor and interferon-γ prevent dexamethasone-induced immunosuppression of antifungal monocyte activity againstAspergillus fumigatushyphae
| Autor: | P.A. Pizzo, Thomas J. Walsh, Andrew Holmes, Cassann Blake, Emmanuel Roilides |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Superoxide
Monocyte General Medicine Biology biology.organism_classification Aspergillus fumigatus Microbiology Respiratory burst chemistry.chemical_compound Infectious Diseases Granulocyte macrophage colony-stimulating factor medicine.anatomical_structure chemistry medicine Interferon gamma MTT assay hormones hormone substitutes and hormone antagonists Dexamethasone medicine.drug |
| Zdroj: | Medical Mycology. 34:63-69 |
| ISSN: | 1460-2709 1369-3786 |
| DOI: | 10.1080/02681219680000101 |
| Popis: | Treatment with corticosteroids is an important risk factor for development of invasive aspergillosis. We evaluated the effect of dexamethasone (DEX) on superoxide anion (O2-) release and damage caused by elutriated human monocytes (EHM) on unopsonized hyphae of Aspergillus fumigatus. In addition, we studied the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon-γ (IFN-γ) on these functions of DEX-treated EHM. Treatment of EHM with concentrations of DEX ranging from 5 to 500 nm (1·4–140 ng ml-1) for 48 h suppressed O2-1 release in response to phorbol myristate acetate in a dose-dependent fashion. Similarly, DEX significantly suppressed hyphal damage caused by EHM as measured by colorimetric MTT assay. Both GM-CSF (5 ng ml-1) and IFN-γ (1·2 ng ml-1) added at day 0 to the EHM together with DEX (500 nm) significantly enhanced O2- release and percentage hyphal damage, preventing the DEX-induced suppression of EHM function. Thus, GM-CSF and IFN-γ prevented the deleterious effect... |
| Databáze: | OpenAIRE |
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