Effects of Nicotine on Human Luteal Cells In Vitro: A Possible Role on Reproductive Outcome for Smoking Women1
| Autor: | Federica Tiberi, Mariateresa Orlando, Francesca Sagnella, Adriano Bompiani, Salvatore Mancuso, Francesca Minici, Rosanna Apa, Fiorella Miceli, Stefania Catino, Anna Tropea, Giuseppina Lamanna, Antonio Lanzone |
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| Rok vydání: | 2005 |
| Předmět: |
endocrine system
medicine.medical_specialty urogenital system Metabolite Cell Biology General Medicine Luteal phase Biology Pathophysiology In vitro Vascular endothelial growth factor Nicotine chemistry.chemical_compound Basal (phylogenetics) Endocrinology Reproductive Medicine chemistry Internal medicine Gene expression medicine reproductive and urinary physiology hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Biology of Reproduction. 72:628-632 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.104.032318 |
| Popis: | We investigated the effect of nicotine and its methylated metabolite, N-methyl-nicotine (M-nicotine), on human luteal cells by measuring release of progesterone and prostaglandins (PGs) from cultured cells and by testing gene expression of vascular endothelial growth factor (VEGF), an angiogenic factor strictly involved in luteal pathophysiology. Primary cultures of human luteal cells were treated for 24 h with nicotine and M-nicotine (from 10−6 to 10−11 M) either alone or combined with hCG (25 ng/ml); progesterone and PGs were assayed in the culture medium. In another group of experiments, luteal cells were treated for 24 h with nicotine and M-nicotine (10−7 M) to perform reverse transcriptase-polymerase chain reaction on VEGF mRNA. Nicotine and M-nicotine negatively affected basal luteal steroidogenesis at all tested concentrations, but neither was able to affect hCG-induced progesterone release. Both substances were able to significantly increase PGF2α release from luteal cells, with a dose-re... |
| Databáze: | OpenAIRE |
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