Development of SCAR markers for aThinopyrum distichumchromosome that appears to be involved in salt tolerance
| Autor: | J. A. Jacobs, G. F. Marais, A. S. Marais, L. Hanekom |
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| Rok vydání: | 2004 |
| Předmět: | |
| Zdroj: | South African Journal of Plant and Soil. 21:236-239 |
| ISSN: | 2167-034X 0257-1862 |
| DOI: | 10.1080/02571862.2004.10635055 |
| Popis: | Sixty-seven F-1 plants from the backcross: Thinopyrum distichum/tetraploid ‘Henoch’ rye//diploid ‘Henoch’ rye were cloned, tested for salt tolerance (2–4 replications per genotype) and ranked. Sixty RAPD primers were tested on the parental lines. Nine of these produced Thinopyrum specific fragments and were applied to the whole population. Correlations between salt tolerance and presence of the Thinopyrum specific bands were mostly significant but small, as would be expected with a trait involving genes on several chromosomes. The nine markers were then also tested on a group of 15 B1 F1 plants from the same cross in which C-banding has identified the Thinopyrum chromosomes. It appeared that OPK6 (± 1060 bp) and OPK17 (± 652 bp) polymorphisms co-segregated with a chromosome putatively identified as belonging to homoeologous group 2 and implied in salt tolerance in a different study. The diagnostic RAPD bands were cloned and sequenced and specific SCAR primers were designed. These amplified DNA sequences s... |
| Databáze: | OpenAIRE |
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