Neuroprotection by trans-resveratrol against collagenase-induced neurological and neurobehavioural deficits in rats involves adenosine A1 receptors
| Autor: | Renu Agarwal, Nafeeza Mohd Ismail, Roqiah Fatmawati Abdul Kadir, Azian Abd Latiff, Igor Nikolayevich Iezhitsa, Noor Azliza Wani Abd Aziz |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Trans-resveratrol business.industry medicine.medical_treatment Antagonist General Medicine Pharmacology Adenosine Neuroprotection nervous system diseases 03 medical and health sciences Adenosine A1 receptor 030104 developmental biology 0302 clinical medicine Neurology Collagenase Medicine cardiovascular diseases Neurology (clinical) business Saline 030217 neurology & neurosurgery medicine.drug Histological examination |
| Zdroj: | Neurological Research. 42:189-208 |
| ISSN: | 1743-1328 0161-6412 |
| DOI: | 10.1080/01616412.2020.1716470 |
| Popis: | Objective: Trans-resveratrol has been shown to have neuroprotective effects and could be a promising therapeutic agent in the treatment of intracerebral haemorrhage (ICH). This study aimed to investigate the involvement of the adenosine A1 receptor (A1R) in trans-resveratrol-induced neuroprotection in rats with collagenase-induced ICH.Methods: Sixty male Sprague-Dawley rats weighing 330-380 g were randomly divided into five groups (n = 12): (i) control, (ii) sham-operated rats, (iii) ICH rats pretreated with vehicle (0.1% DMSO saline, i.c.v.), (iv) ICH rats pretreated with trans-resveratrol (0.9 µg, i.c.v.) and (v) ICH rats pretreated with trans-resveratrol (0.9 µg) and the A1R antagonist, DPCPX (2.5 µg, i.c.v.). Thirty minutes after pretreatment, ICH was induced by intrastriatal injection of collagenase (0.04 U). Forty-eight hours after ICH, the rats were assessed using a variety of neurobehavioural tests. Subsequently, rats were sacrificed and brains were subjected to gross morphological examination of the haematoma area and histological examination of the damaged area.Results: Severe neurobehavioural deficits and haematoma with diffuse oedema were observed after intrastriatal collagenase injection. Pretreatment with trans-resveratrol partially restored general locomotor activity, muscle strength and coordination, which was accompanied with reduction of haematoma volume by 73.22% (P < 0.05) and damaged area by 60.77% (P < 0.05) in comparison to the vehicle-pretreated ICH group. The trans-resveratrol-induced improvement in neurobehavioural outcomes and morphological features of brain tissues was inhibited by DPCPX pretreatment.Conclusion: This study demonstrates that the A1R activation is possibly the mechanism underlying the trans-resveratrol-induced neurological and neurobehavioural protection in rats with ICH. |
| Databáze: | OpenAIRE |
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