Effect of tridemorph and fenpropimorph on sterol composition in fenugreek
| Autor: | Alain Rahier, Yves Sauvaire, Claude Cerdon, Maryse Taton |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Fenpropimorph Plant Science General Medicine Horticulture Biology Biochemistry Sterol Fungicide chemistry.chemical_compound Enzyme chemistry Tridemorph Biosynthesis Cell culture polycyclic compounds lipids (amino acids peptides and proteins) Composition (visual arts) Molecular Biology |
| Zdroj: | Phytochemistry. 41:423-431 |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/0031-9422(95)00550-1 |
| Popis: | The addition of tridemorph (125 μM) during growth of fenugreek seedlings or the addition of fenpropimorph (125 μM) in the culture medium of fenugreek cell suspensions resulted in a large accumulation of 9β,19-cyclopropyl sterols (about 50% of total sterols) concomitant with a decrease in the Δ5-sterol content (40–50% of total sterols compared with more than 90% in control cultures). However the 9β,19-cyclopropyl sterols accumulated differed depending on the tissue studied—fenugreek roots or cells. In roots of seedlings treated with tridemorph, pollinastanol and 4α,14α-dimethyl-9β,19-cyclo-5α-cholestan-3β-ol were the major cyclopropyl sterols while in treated-cell suspensions with fenpropimorph, 24-methylenepollinastanol and 24-methylpollinastanol were the major sterols. These results indicated that these compounds inhibited the cycloeucalenol-obtusifoliol-isomerase (COI). Moreover, in roots as well as in cell suspensions, these inhibitors led to a weak accumulation of Δ8-sterols indicating that Δ8 → Δ7 isomerase was also affected. Finally, we observed that only fenpropimorph induced an accumulation of Δ8, 14-sterols in cell suspensions, showing that Δ14-reductase can also be inhibited by this fungicide. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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