| Popis: |
Salmonella Typhi is one of the most common enteric pathogens in both humans and animals that cause by the consumption of contaminated water and foods. In silico tools are used to model a stable and reliable vaccine against such infections. In the present study, a vaccine based on the outer-membrane proteins in Salmonella Typhi, has been designed. CD4 and CD8 epitopes and B-cells were predicted. To develop the final vaccine, all epitopes were joined together by AAY, KK, and GPGPG linkers. A potent immune adjuvant known as Heparin-Binding Hemagglutinin Adhesin (HBHA) has incorporated into the N-terminal of the final vaccine. Physicochemical characteristics, antigenicity, allergenicity, and immunogenicity of construct epitopes were defined using online tools. Finally; Molecular docking of developed vaccine constructs was done with TLR4-receptor. ∆G of refined vaccine with TLR4-receptor is -3.91×10 Kcal mol with 1.93 RMSD. Probability antigenicity of CD4 and CD8 epitopes were determined. The physicochemical assay classified the protein as stable. The probability antigenic vaccine is a safe vaccine according to results that indicate that vaccine is non-allergen. In conclusion, the multi-epitope vaccine might be considered to combat typhoid fever. |
