Assessment of caspase activity as a possible prognostic factor in acute myeloid leukaemia

Autor:	Lisa Oliver, François-Marie Vallette, Jean-Luc Harousseau, Khaled Meflah, Pascale Perrin, Fabienne Vavasseur, Beatrice Mahe
Rok vydání:	2002
Předmět:	biology Effector Chemistry Hematology Cleavage (embryo) Multiple drug resistance medicine.anatomical_structure Apoptosis hemic and lymphatic diseases Precursor cell Immunology biology.protein Cancer research medicine DNA fragmentation Bone marrow Caspase
Zdroj:	British Journal of Haematology. 118:434-437
ISSN:	0007-1048
DOI:	10.1046/j.1365-2141.2002.03640.x
Popis:	Summary. Caspases are one of the key effector molecules in apoptosis. Caspase-3 activity (identified by cleavage of the peptide DEVD) was analysed in bone marrow blasts (minimum 70%) from 45 acute myeloid leukaemia (AML) patients. 12 patients (25%) exhibited high levels of specific DEVDase activity. These blast cells, despite having activated caspase-3, displayed none of the classical caspase-dependent morphological characteristics of apoptosis, such as degradation of DNA fragmentation factor 45, DNA fragmentation, and appeared to be more resistant to drug-induced apoptosis. Our results suggest that in these AML cells, resistance to apoptosis occurred downstream of caspase-3 activation.
Databáze:	OpenAIRE
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