Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetic Sifter)
| Autor: | Dawson J. Wong, Sanjiv S. Gambhir, Chin Chun Ooi, Seung-min Park, Shan X. Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chemistry medicine.medical_treatment Magnetic separation medicine.disease Primary tumor Targeted therapy Gene expression profiling 03 medical and health sciences 030104 developmental biology Circulating tumor cell Single-cell analysis Cancer research medicine Liquid biopsy Whole blood |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493971435 |
| DOI: | 10.1007/978-1-4939-7144-2_12 |
| Popis: | Circulating tumor cells (CTCs) are currently widely studied for their potential application as part of a liquid biopsy. These cells are shed from the primary tumor into the circulation, and are postulated to provide insight into the molecular makeup of the actual tumor in a minimally invasive manner. However, they are extremely rare in blood, with typical concentrations of 1-100 in a milliliter of blood; hence, a need exists for a rapid and high-purity method for isolating CTCs from whole blood. Here, we describe the application of a microfabricated magnetic sifter toward isolation of CTCs from whole blood at volumetric flow rates of 10 mL/h, along with the use of a PDMS-based nanowell system for single-cell gene expression profiling. This method allows rapid isolation of CTCs and subsequent integration with downstream genetic profiling methods for clinical applications such as targeted therapy, therapy monitoring, or further biological studies. |
| Databáze: | OpenAIRE |
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