Wearable materials with embedded synthetic biology sensors for biomolecule detection
| Autor: | James J. Collins, James B. Niemi, Shimyn Slomovic, Tommaso Galbersanini, Hani M. Sallum, Nicolaas M. Angenent-Mari, Ally Huang, Rose A. Lee, Geoffrey Lansberry, Helena de Puig, Luis R. Soenksen, Peter Q. Nguyen, Nina M. Donghia, Evan M. Zhao |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Laboratory methods Computer science business.industry Biomolecule Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Biomedical Engineering Wearable computer Bioengineering Nanotechnology Applied Microbiology and Biotechnology 03 medical and health sciences Synthetic biology 0302 clinical medicine chemistry Molecular targets Molecular Medicine CRISPR business 030217 neurology & neurosurgery Wearable technology 030304 developmental biology Biotechnology |
| Zdroj: | Nature Biotechnology. 39:1366-1374 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/s41587-021-00950-3 |
| Popis: | Integrating synthetic biology into wearables could expand opportunities for noninvasive monitoring of physiological status, disease states and exposure to pathogens or toxins. However, the operation of synthetic circuits generally requires the presence of living, engineered bacteria, which has limited their application in wearables. Here we report lightweight, flexible substrates and textiles functionalized with freeze-dried, cell-free synthetic circuits, including CRISPR-based tools, that detect metabolites, chemicals and pathogen nucleic acid signatures. The wearable devices are activated upon rehydration from aqueous exposure events and report the presence of specific molecular targets by colorimetric changes or via an optical fiber network that detects fluorescent and luminescent outputs. The detection limits for nucleic acids rival current laboratory methods such as quantitative PCR. We demonstrate the development of a face mask with a lyophilized CRISPR sensor for wearable, noninvasive detection of SARS-CoV-2 at room temperature within 90 min, requiring no user intervention other than the press of a button. |
| Databáze: | OpenAIRE |
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