Silver- and gold-labeled colloidal and crosslinked glycopolymers based on glycyl glycosynthons and maleic anhydride copolymers for lectin binding
| Autor: | Leonid M. Likhosherstov, N. A. Samoilova, V. E. Piskarev, Igor A. Yamskov, M. A. Krayukhina, T. A. Babushkina |
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| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Aqueous solution Materials science Polymers and Plastics biology Lectin Maleic anhydride 02 engineering and technology General Chemistry Polymer 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Silver nanoparticle 0104 chemical sciences Surfaces Coatings and Films chemistry.chemical_compound Colloid chemistry Colloidal gold Polymer chemistry Materials Chemistry biology.protein Copolymer 0210 nano-technology |
| Zdroj: | Journal of Applied Polymer Science. 134 |
| ISSN: | 0021-8995 |
| Popis: | A simple, convenient, and inexpensive technique for preparing lectin-specific nanogold- and silver-labeled colloidal and crosslinked neoglycoconjugates, based on maleic anhydride copolymers, was developed. Water-soluble nanogold- and silver-labeled glyconanoparticles were obtained in two steps: (1) introduction of free N-acetyl-D-glucosamine or glycyl-spacered glycosynthons—β-N-glycyl-N-acetyl-D-glucosamine or β-N-glycyl-lactose—into poly(ethylene-alt-maleic anhydride) or poly(N-vinylpyrrolidone-alt-maleic anhydride) and (2) labeling in situ the thus-obtained glycopolymers with gold or silver nanoparticles. Lectin sorbents were synthesized from glycyl-spacered glycosynthons and spherical, granulated, crosslinked maleic anhydride copolymers in an aqueous system without any condensing agents. Thus, maleic anhydride copolymer was used solely, without any preliminary modification of the polymer, as a matrix for crosslinking, for specific ligand binding, and as metal nanoparticles as a stabilizing cover. The resulting colloidal gold or silver glyconanoparticles were used as lectin sensors (in a dot-blot analysis of lectins) and as crosslinked neoglycoconjugates for lectin sorption studies. The corresponding gold- or silver-labeled water-soluble glyconanoparticles and crosslinked neoglycoconjugates manifested high activity and specificity in all tests with a series of β-D-GlcNAc-specific and β-D-Gal-specific lectins. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44718. |
| Databáze: | OpenAIRE |
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