Ultrastructural Concomitants of Sodium Butyrate-Enhanced Ectopic Production of Chorionic Gonadotropin and Its Alpha Subunit in Human Bronchogenic Carcinoma (ChaGo) Cells
| Autor: | Bruce D. Weintraub, Tommie Sue Tralka, Alan S. Rabson, Bruce K. Wetzel, Elizabeth W. Kingsbury, Jeffrey M. Lieblich, Linda W. Engel, Saul W. Rosen |
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| Rok vydání: | 1979 |
| Předmět: |
endocrine system
Cancer Research medicine.medical_specialty medicine.drug_class Endoplasmic reticulum Immunocytochemistry Sodium butyrate Golgi apparatus Biology Cell biology chemistry.chemical_compound symbols.namesake Endocrinology Oncology chemistry Cytoplasm Internal medicine symbols medicine Ultrastructure Gonadotropin G alpha subunit |
| Zdroj: | JNCI: Journal of the National Cancer Institute. |
| ISSN: | 1460-2105 |
| DOI: | 10.1093/jnci/62.1.45 |
| Popis: | Sodium butyrate treatment of cultures of ChaGo (human lung cancer) cells resulted in increased production of human chorionic gonadotropin (hCG) and its alpha subunit (hCG-alpha) and induced a variety of morphologic changes. Elongation and flattening of cells were seen by light microscopy. Immunocytochemistry with antisera against hCG and against hCG-alpha showed an increase in cells containing stainable hCG-alpha. Scanning electron microscopy demonstrated enhanced adhesion of cells to glass cover slips, with elongation, flattening, and decreased cytoplasmic blebs. Ultrastructural changes were examined by transmission electron microscopy and evaluated quantitatively by an unbiased observer. Significant findings included increases in perinuclear tonofilaments, smooth endoplasmic reticulum vesicles, dense mitochondrial inclusions, and lipid granules, as well as decreases in intercellular desmosomes, free polyribosomes, mitochondrial dense granules, and Golgi complexes. The most notable change, a marked decrease in condensed chromatin clumps, may have reflected a butyrate-induced biochemical modification of chromatin leading to enhanced accessibility of certain genes for transcription. |
| Databáze: | OpenAIRE |
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