Abstract 12516: Vascular Calcification Does Not Affect Cardiovascular Structure and Function During the Reversal of Atherosclerosis
| Autor: | Daniel M Kalta, Xin Lin, Mohnish Singh, Olga V Savinova |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Circulation. 144 |
| ISSN: | 1524-4539 0009-7322 |
| DOI: | 10.1161/circ.144.suppl_1.12516 |
| Popis: | Introduction: Animal models are used to study the mechanisms of reversal of atherosclerosis. Due to the minimal extent of plaque calcification, commonly used mouse models do not provide good insight into the regression of calcified plaques. To address this question, we developed a mouse strain that is genetically predisposed to atherosclerotic calcification. Hypothesis: The purpose of this study was to compare the effects of the reversal of atherosclerosis on cardiovascular physiology between mice predisposed to calcification and control mice. Methods: All mice (n=35) had a mutation of a low-density lipoprotein receptor and developed atherosclerosis over a course of 15 weeks while on a Western diet. The experimental groups consisted of mice overexpressing TNAP (tissue-nonspecific alkaline phosphatase) in macrophages under the control of LysM-Cre recombinase, and the control littermates. After 23 weeks of age (baseline), mice were switched to a normal diet. Echocardiographic studies were performed to assess cardiac structure and function at baseline and at 52 weeks of age at the completion of the diet reversal protocol. MicroCT analysis of calcification was performed ex vivo . Data were analyzed via 2-way ANOVA to calculate the effect of diet (atherosclerotic baseline vs reversal) while adjusting for sex. Results: Cardiac parameters including left ventricular (LV) mass, LV internal diameter and wall thickness, ejection fraction, fractional shortening, and aortic valve flow velocity were within the normal range and not statistically different between the two genotypes. CT data confirmed an increase in calcium volume in TNAP overexpressor mice compared to controls. Conclusions: Our data suggest that under conditions of the reversal of atherosclerosis, plaque calcification at baseline is not associated with the deterioration of cardiac structure and function in a mouse model. |
| Databáze: | OpenAIRE |
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