| Autor: |
Tianxiang Ma, Xiao Liu, Haoran Su, Yuan He, Fan Wu, Chenxing Gao, Kexin Li, Zhuqing Liang, Dongrui Zhang, Xing Zhang, Ke Hu, Shangyu Li, Li Wang, Min Wang, Shuhua Yue, Weili Hong, Xun Chen, Jing Zhang, Xiaoyan Deng, Pu Wang, Yubo Fan |
| Rok vydání: |
2022 |
| Popis: |
Mechanical forces, including flow shear stress, regulate fundamental cellular process by modulating the nucleocytoplasmic transport of transcription factors, such as Yes-associated Protein (YAP). However, the mechanical mechanism how flow induces the nucleocytoplasmic transport remains largely unclear. Here we found that unidirectional flow applied to endothelial cells induces biphasic YAP nucleocytoplasmic transport with initial nuclear import, followed by nuclear export as perinuclear actin cap forms and nuclear stiffening in a dose and timing-dependent manner. In contrast, pathological oscillatory flow induces slight actin cap formation and nuclear softening, sustaining YAP nuclear localization. To explain the disparately spatiotemporal distribution of YAP, we developed a three-dimensional mechanochemical model considering coupling processes of flow sensing, cytoskeleton organization, nucleus mechanotransduction, and YAP spatiotemporal transport. We discovered that actin cap formation and nuclear stiffness alteration under flow synergically regulate nuclear deformation, hence governing YAP transport. Furthermore, we expanded our single cell model to a collective vertex framework and found that actin cap irregularities in individual cells under flow shear stress potentially induce topological defects and spatially heterogeneous YAP distribution in cellular monolayers. Our work unveils the unified mechanism of flow-induced nucleocytoplasmic transport, offering a universal linkage between transcriptional regulation and mechanical stimulation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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