Rapid direct resolution of the stereoisomers of all-trans astaxanthin on a Pirkle covalent l-leucine column
| Autor: | Saleh A. Turujman |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Chromatography
Chemistry Stereochemistry Meso compound Organic Chemistry General Medicine Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Pigment Astaxanthin visual_art visual_art.visual_art_medium Racemic mixture Leucine Enantiomer Cis–trans isomerism |
| Zdroj: | Journal of Chromatography A. 631:197-199 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/0021-9673(93)80521-9 |
| Popis: | Astaxanthin, 3,3′dihydroxy-β,β-carotene-4,4′-dione, gives salmon flesh its distinctive color. Synthetic all- trans astaxanthin consists of a racemic mixture of two enantiomers (3 R ,3′ R and 3 S ,3′ S ) and a meso form (3 R ,3′ S ). The stereoisomeric composition of endogenous astaxanthin in wild salmon differs from that of synthetic astaxanthin added to the fish feed of pond-bred salmon. In order to determine the origin of astaxanthin in salmon flesh, a method is needed that can distinguish the various isomeric forms. HPLC conditions are described for the rapid direct resolution of the three stereoisomers of all- trans astaxanthin on a Pirkle covalent l -leucine column. This method also partially resolves the stereoisomeric forms of the major cis isomer. |
| Databáze: | OpenAIRE |
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