Subcellular Compartmentation of Metabolites Involved in Cold Acclimation
| Autor: | Imke I. Hoermiller, Thomas Nägele, Arnd G. Heyer |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Metabolite fungi food and beverages Vacuole Mitochondrion Plant cell 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Cytosol 030104 developmental biology chemistry Biochemistry Cold acclimation Cell fractionation Plastid 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071606599 |
| DOI: | 10.1007/978-1-0716-0660-5_18 |
| Popis: | Plant cells are heavily compartmentalized, and metabolite concentrations in the various compartments differ significantly. Thus, determination of metabolite abundance in whole-cell extracts may be misleading, when the role of a compound in plant freezing tolerance shall be evaluated. Here, we describe a method for the separation of the largest compartments of plant cells, the vacuole, plastid, and cytosol. With more elaborate analysis, this method can be expanded to also resolve mitochondria and other compartments. |
| Databáze: | OpenAIRE |
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