Effects of exosome-like vesicles on cumulus expansion in pigs in vitro
| Autor: | Wataru Fujii, Koji Sugiura, Kunihiko Naito, Kazuma Yasuhara, Asuka Onuma, Yoshie A. Fujioka, Yuta Matsuno |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Messenger RNA Cell signaling 030219 obstetrics & reproductive medicine Chemistry Vesicle RNA Follicular fluid Exosome In vitro Microvesicles Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Endocrinology Internal medicine medicine Animal Science and Zoology |
| Zdroj: | Journal of Reproduction and Development. 63:51-58 |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.2016-124 |
| Popis: | Cell-secreted vesicles, such as exosomes, have recently been recognized as mediators of cell communication. A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in other mammalian species is not known. Therefore, this study aimed to examine the presence of exosome-like vesicles in ovarian follicles and their effects on cumulus expansion in vitro in pigs. The presence of exosome-like vesicles in porcine follicular fluid (pFF) was confirmed by transmission electron microscopic observation, the detection of marker proteins, and RNA profiles specific to exosomes. Fluorescently labeled exosome-like vesicles isolated from pFF were incorporated into both cumulus and mural granulosa cells in vitro. Exosome-like vesicles were not capable of inducing cumulus expansion to a degree comparable to that induced by follicle-stimulating hormone (FSH). Moreover, exosome-like vesicles had no significant effects on the expression levels of transcripts required for the normal expansion process (HAS2, TNFAIP6, and PTGS2). Interestingly, FSH-induced expression of HAS2 and TNFAIP6 mRNA, but not of PTGS2 mRNA, was significantly increased by the presence of exosome-like vesicles; however, the degree of FSH-induced expansion was not affected. In addition, porcine exosome-like vesicles had no significant effects on the expansion of mouse cumulus-oocyte complexes. Collectively, the present results suggest that exosome-like vesicles are present in pFF, but they are not efficient in inducing cumulus expansion in pigs. |
| Databáze: | OpenAIRE |
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