In Vitro Culture Conditions for Reproductive Endocrine Tissues of Fathead Minnow (Pimephales promelas)

Autor: Perkins E, Morgan Re, Johnston T, Ferguson Dc, Cropek Dm
Rok vydání: 2014
Předmět:
Zdroj: Cell & Developmental Biology.
ISSN: 2168-9296
DOI: 10.4172/2168-9296.1000146
Popis: Studies examined the effects of different culture conditions for the brain, gonads (testis and ovary), and liver of adult male and Female Fathead Minnow (Pimephales promelas) (FHM) during a 28 day assessment of viability and functionality. Five parameters were tested including media pH, incubation temperature, media composition, media exchange rate, and substrate. Within the ranges tested, incubation temperature was the most influential on tissue viability with dramatic improvements using cooler incubation temperatures (
Databáze: OpenAIRE