| Popis: |
The objective of the presented study was to find out whether the leukocytes in the platelet clot affect the healing of a complicated wound. The study was conducted on Wistar rats. Before the wound was inflicted, the animals were immersed in sevoflurane anesthesia. Wounds were inflicted in the interscapular area with a scalpel preheated in the flame of a gas burner to about 300.00 C. Three days after the wound was inflicted, in order to reduce the amount of cellular detritus, enzymatic necroectomy was performed first by 60-minute applications of trypsin solution (10.0 mg /ml), and then surgical necroectomy. The animals were divided into 4 groups: control group No. 1 (wounds were not sutured), control group No. 2 (wounds were sutured, but without the introduction of a fibrin clot), experimental group No. 1 (wounds were sutured after placing a platelet clot in them), experimental group No. 2 (wounds were sutured after placing a platelet clot in themleukocyte clot). According to the results of the study, it was shown that in both experimental groups, the healing of the wound defect occurred faster than in the control. However, when comparing the healing effects of pure platelet and plateletleukocyte clots, the result was ambiguous. A platelet-leukocyte clot, in comparison with a purely platelet clot, somewhat slowed down wound healing by inducing early rejection of sutures and slowing down scar remodeling. According to the results of the study, it can be concluded that the leukocytes in the clot can actually clear the wound. But in the case of application of such a clot to a wound, the surface of which was previously subjected to intensive necroectomy, its leukocyte component can direct its pro-inflammatory effect not on pathogenic microflora and necrotic detritus, but on healthy tissues. Thereby inducing hyperergic inflammation and slowing healing. The obtained result suggests that the study should be expanded, using wounds of varying degrees of infection and with varying degrees of necrosis in the wound canal area as models. |
