Increased enzyme activity in immobilised cell composites with a highly hydrophilic polymer matrix

Autor:	Isao Kaetsu, Minoru Kumakura
Rok vydání:	2008
Předmět:	chemistry.chemical_classification Glucose-6-phosphate isomerase Acrylate Chemical structure General Engineering General Medicine Polymer Methacrylate Michaelis–Menten kinetics chemistry.chemical_compound Monomer chemistry Polymerization Polymer chemistry Composite material
Zdroj:	Journal of Chemical Technology and Biotechnology. Biotechnology. 34:39-44
ISSN:	1935-1828 0264-3421
DOI:	10.1002/jctb.280340107
Popis:	Streptomyces phaeochromogenes cells were immobilised by radiation polymerisation of hydrophilic monomers; highest levels of glucose isomerase activity were found in immobilised cell composites with highly hydrophilic polymer matrices. The enzyme activity in the composites from the copolymerisation of hydroxyethyl acrylate (or methacrylate) and N-vinyl-2-pyrrolidone monomer increased markedly with increase in the content of the N-vinyl-2-pyrrolidone monomer, owing to the higher water absorption by the polymer matrix, and was close to that of the non-immobilised cells. The enzyme activity was affected by monomer concentration, the hydrophilicity, and chemical structure of the polymer matrix. The Michaelis constant of the composites from hydrophilic monomers was close to that of the intact cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::080962cfce24a0a3aeb3e81dded0677a https://doi.org/10.1002/jctb.280340107 Zobrazit plný text záznamu