Characterization of Na+, K+ ATPase in erythrocytes of dairy cows: Effect of diet and genetic selection for milk solids

Autor:	N. D. Scollan, Brian W. McBride, N. S. Jessop, J. R. Brisbane, J. D. Oldham, G. F. Allan, Xin Zhao
Rok vydání:	1994
Předmět:	chemistry.chemical_classification ATPase Forage Biology Enzyme medicine.anatomical_structure Food Animals chemistry Lactation medicine biology.protein Genetic selection Animal Science and Zoology Dry matter Food science Na+/K+-ATPase Receptor
Zdroj:	Canadian Journal of Animal Science. 74:641-647
ISSN:	1918-1825 0008-3984
DOI:	10.4141/cjas94-093
Popis:	The number and activity of Na+, K+ ATPase units were assessed with 3H-ouabain binding and 86Rb+ uptake methods, respectively, in erythrocytes from second-parity Holstein–Friesian cows at weeks 10 and 24 of lactation. The cows were either from S or C genetic lines for yield of milk fat and protein and were offered diets of either H or L forage content. The Scatchard method of analysis indicated that 3H-ouabain binding was compatible with a single class of receptors of high affinity. S-line cows had higher milk yields (P d) and the number and activity of Na+, K+ ATPase units. The number of enzyme units was higher at week 24 than at week 10 (P 86Rb+ uptake for groups CL and SH may reflect an imbalance between genotype and nutrient provision. Positive relationships were found between the number and activity of Na+, K+ ATPase units and the milk energy output (P +, K+ pumping that is required for enhanced milk productivity and suggests that the activity of erythrocyte Na+, K+ ATPase reflects the activity of this enzyme in other tissues during lactation. Key words: Dairy cows, genotype, diet, erythrocyte, Na+, K+ ATPase
Databáze:	OpenAIRE
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