Immunochemical analysis of the distribution of the new ovary maturating neurohormone during development of the African locust,Locusta migratoria
| Autor: | Josiane Girardie, Olivier Richard |
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| Rok vydání: | 1992 |
| Předmět: |
Antiserum
medicine.medical_specialty Histology biology Immunocytochemistry Embryo Ovary Cell Biology biology.organism_classification Pathology and Forensic Medicine Blot Endocrinology medicine.anatomical_structure Polyclonal antibodies Internal medicine biology.protein medicine Neurosecretion Locust |
| Zdroj: | Cell and Tissue Research. 270:587-596 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/bf00645062 |
| Popis: | Following our prior identification of a gonadotropic neurohormone isolated from the neurosecretory lobe of the corpora cardiaca of the African locust, we have raised a polyclonal antiserum against this new molecule. In the present paper, we characterize this antiserum using enzyme-linked immunosorbent assay and Western blotting. The latter procedure reveals that the immune serum specifically recognizes the neurohormone, which we have termed ‘ovary maturating parsin’. Immunohistochemistry, enzyme-linked immunosorbent assay and Western blotting were used to analyze the distribution of this gonadotropic neurohormone throughout the central nervous system during development. It is produced only by the type-B neurosecretory cells of the pars intercerebralis-corpora cardiaca system and is present both in males and females throughout life from embryo to adult. This permanent expression suggests that the neurohormone may have functions other than its primary direct gonadotropic role in females. |
| Databáze: | OpenAIRE |
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