| Popis: |
Two Gram-negative, moderately halophilic, and motile rod bacteria, strains G2-23T and J2-29T, showing catalase- and oxidase-positive activities were isolated from marine algae. Both strains optimally grew at 30°C, pH 7.0, and 2% (w/v) NaCl. Both strains contained ubiquinone-10 as the sole isoprenoid quinone. As major cellular fatty acids and polar lipids, strain G2-23T contained summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, and summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c/ω6c) and phosphatidylethanolamine (PE), phosphatidyl-N-monomethylethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol, sulfoquinovosyl diacylglycerol (SQDG), and an unidentified phospholipid (PL), respectively, while strain J2-29T contained summed feature 8, C18:1 ω7c 11-methyl, and C16:0 and PE, PME, PG, SQDG, and PL, respectively. The genomic DNA G + C contents of strains G2-23T and J2-29T were 59.5 and 62.2 mol%, respectively. Both strains shared 97.9% 16S rRNA gene sequence similarity, 79.8% average nucleotide identity (ANI), and 22.8% digital DNA-DNA hybridisation (DDH) value, indicating that they are different species. Phylogenetic and phylogenomic analyses by 16S rRNA gene and genome sequences, respectively, revealed that strains G2-23T and J2-29T formed different phylogenic lineages within the genus Hoeflea. ANI and digital DDH values between strains G2-23T and J2-29T and other Hoeflea type strains were less than 79.0 and 22.1% and 80.5 and 23.3%, respectively, representing that they are novel species of the genus Hoeflea. Based on the phenotypic, chemotaxonomic, and molecular properties, strains G2-23T and J2-29T represent two different novel species of the genus Hoeflea, for which the names Hoeflea algicola sp. nov. (G2-23T = KACC 22714T = JCM 35548T) and Hoeflea ulvae sp. nov. (J2-29T = KACC 22715T = JCM 35549T), respectively, are proposed. |
