| Popis: |
Ceramide, mono- and diglycosylceramide were isolated from spinach leaves, and their components and principal molecular species were studied. The major fatty acid components were 2-hydroxylignoceric and 2-hydroxybehenic acids in ceramide and diglycosylceramide, and 2-hydroxypalmitic and 2-hydroxylignoceric acids in monoglycosylceramide. As component sphingoids at least eight species of dihydroxy and trihydroxy bases were commonly found. The principal sphingoid components of ceramide were 4-hydroxy-8-sphingenine and 4-hydroxysphinganine, whereas those of glycosylceramides were 4,8-sphingadienine and 4-hydroxy-8-sphingenine. The sugar component of monoglycosylceramide was only glucose, but in diglycosylceramide glucose and mannose were found in the ratio of 85:15. The main ceramide species was N-2′-hydroxylignoceroy1-4-hydroxy-8-sphingenine. Generally, monoglycosylceramide consisted of two types of component ceramide species, viz., hydroxypalmitoyl-dihydroxysphingoid and hydroxylignoceroyl-trihydroxysphingoid. The principal species of monoglycosylceramide were found to be 1-O-β-glucosyl-N-2′-hydroxypalmitoyl-4, 8-sphingadienine and 1-O-β-glucosyl-N-2′-hydroxylignoceroyl-4-hydroxy-8-sphingenine. The major species of diglycosylceramide was postulated as 1-O-β-cellobiosyl-N-2′-hydroxylignoceroyl-4-hydroxy-8-sphingenine. |
