Cloning and Expression of Lipopolysaccharide Elimination Protein (LEP) in Lactic Acid Bacteria
| Autor: | Kyoko Asami, Makoto Kanauchi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Cloning 0303 health sciences biology Brevibacillus education 04 agricultural and veterinary sciences medicine.disease_cause biology.organism_classification 040401 food science Lactic acid Amino acid 03 medical and health sciences chemistry.chemical_compound Transformation (genetics) 0404 agricultural biotechnology Biochemistry chemistry Heat shock protein medicine Escherichia coli hormones hormone substitutes and hormone antagonists health care economics and organizations Bacteria 030304 developmental biology |
| Zdroj: | Lactic Acid Bacteria ISBN: 9781493989065 |
| DOI: | 10.1007/978-1-4939-8907-2_16 |
| Popis: | Lipopolysaccharide (LPS) is related to human inflammation. Therefore, in the probiotics research field, controlling the mechanisms of LPS neutralization and elimination of inflammation of human intestines are important. This chapter presents a description of the identification of LPS elimination protein (LEP) in lactic acid bacteria (LAB), cloning of its protein, and its expression. First, LEP is extracted from the LAB cell wall digestion fraction using Blue Native PAGE. Then LEP is identified by the elimination activity of LPS on gel pieces. Results show that the LEP is an approx. 200 kDa protein part of heat shock protein in lactic acid bacteria. After sequencing amino acids of LEP, LEP cloning is done using a Brevibacillus sp. expression system without a general transformation system but with Gram-negative Escherichia coli having LPS. Results presented in this chapter demonstrate the elimination activity of recombinant LEP. |
| Databáze: | OpenAIRE |
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