Enzyme activities in the integument of amphibia: Biochemical, histochemical and electrophysiological data onRanakl.esculentaandXenopus laevis
| Autor: | Tiziana Ravasenga, Rossella Barberis, Bruno Emilio Dore, Daniela Donna, Enrico Vinai, Gian Emilio Andreoletti |
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| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
biology Xenopus Periodic acid Periodic acid–Schiff stain Apical membrane biology.organism_classification Enzyme assay Rana chemistry.chemical_compound Enzyme medicine.anatomical_structure chemistry Biochemistry Biophysics biology.protein medicine Animal Science and Zoology Epidermis |
| Zdroj: | Italian Journal of Zoology. 71:63-68 |
| ISSN: | 1748-5851 1125-0003 |
| DOI: | 10.1080/11250000409356608 |
| Popis: | The enzymatic activities related to Rana kl. esculenta and Xenopus laevis skin, and in particular to an unstirred layer in the epidermis surface (subcorneal space) where mitochondria‐rich cells (MR cells) and principal cells (PC cells) expose their apical membrane, are studied; histochemical, biochemical and electro‐physiological data are compared. The organization of PAS (Periodic Acid Schiff)/PASM (Periodic Acid, Silver Methenamine) positive material and some ectoenzymes in the subcorneal space and in the apical surface of living and active cells, both MR and PC are described. Subcorneal space appears to be a specialized structure, forming an unstirred layer at the limiting surface of amphibians living in water. This layer may function as an efficient barrier to capture ions and small molecules diffusing from living cells in the skin, so forming the space where active reuptake of ions and small diffusible organic molecules may take place, but in thermo‐dynamically favourable conditions. |
| Databáze: | OpenAIRE |
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