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ObjectivesThis study aimed to identify SPARC expression in laryngeal carcinoma and its relationship with the tumor immune microenvironment at the genomic level.MethodsThe data of SPARC expression for laryngeal carcinoma tumor tissues and adjacent normal tissues was downloaded from TCGA database. Then, CIBERSORT was used to deconvolve the proportion of 22 types of immune cells in laryngeal carcinoma normal tissues and cancer tissues. The GO and KEGG pathway enrichment analysis of the Hub genes that were co-expressed with SPARC were performed using R package. The Kaplan-Meier and log-rank test were used to perform survival analysis. ResultsThe SPARC expression in laryngeal carcinoma tumor tissues was elevated relative to normal tissue (P < 0.001). Similarly, compared with paired adjacent normal tissues, SPARC expression significantly upregulated in tumor tissues (P = 0.002). However, survival analysis showed that there was no significant different in overall survival (OS) between SPARC low expression group and high expression group (P = 0.952). SPARC was negatively associated with the infiltration of B cells memory (r = -0.24, P = 0.024) and plasma cells (r = -0.26, P = 0.014). In addition, SPARC was positively correlated with macrophages M0 (r = 0.22, P = 0.038) and T cells CD4 memory resting (r =0.26, P = 0.014). Furthermore, the Hub genes of SPARC were enriched in extracellular matrix structural constituent, cell growth and/or maintenance and Beta3 integrin cell surface interactions.ConclusionsSPARC was overexpressed in laryngeal carcinoma tissues compared with normal adjacent tissues and was correlated with immune cells infiltrating. |
