Autor:	T. B. Krokhina, S. M. Terekhov, Elizaveta S. Ershova, S. S. Shishkin, I. N. Krakhmaleva, S. F. Zakharov
Rok vydání:	2001
Předmět:	Biological studies Myogenesis Genetic enhancement Biological activity Biology musculoskeletal system medicine.disease Molecular biology General Biochemistry Genetics and Molecular Biology medicine Myocyte Bioassay Stem cell Muscular dystrophy General Agricultural and Biological Sciences
Zdroj:	Biology Bulletin of the Russian Academy of Sciences. 28:630-635
ISSN:	1062-3590
DOI:	10.1023/a:1012380505528
Popis:	The method for obtaining human myoblast culture has been modified to consider the specific histological localization of the satellite cells as well as their growth properties; the cultivation conditions have been selected to grow up to 150000 cells/cm2. At high densities, the cells remain mononuclear and preserve their typical myoblast morphology as well as the capacity for fusion and the formation of myotubes. By contrast to fibroblasts, up to 80% of the cells in the myoblast culture were positive in the acid phosphatase test, which indicates their stem nature. The obtained myoblast cultures were used in the clinical tests of cell-mediated gene therapy of Duchenne's muscular dystrophy as well as in the bioassay for the effects of biologically active compounds.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::049b7f9456989962e80f2d3003b9e038 https://doi.org/10.1023/a:1012380505528 Zobrazit plný text záznamu Full text from SpringerLink