Measurement of lipase activity in rubber (Hevea brasiliensis ) seed
Autor: | Obioma U. Njoku, I. C. Ononogbu, F. U. Eneh |
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Rok vydání: | 1996 |
Předmět: |
chemistry.chemical_classification
biology General Chemical Engineering Organic Chemistry Euphorbiaceae Triacylglycerol lipase food and beverages Fatty acid biology.organism_classification medicine.disease chemistry Natural rubber Biochemistry visual_art biology.protein visual_art.visual_art_medium medicine Lipolysis Hevea brasiliensis Food science Dehydration Lipase |
Zdroj: | Journal of the American Oil Chemists' Society. 73:1471-1473 |
ISSN: | 0003-021X |
DOI: | 10.1007/bf02523512 |
Popis: | Lipase activity in para rubber (Hevea brasiliensis) seed was measured by monitoring the release of free fatty acid by lipolysis of the endogenous lipid in a crushed sample of seed incubated at 37.5°C for 30 min. Free fatty acid was determined colorimetrically by a modified copper soap method. Fresh seeds showed the highest lipase activity. Drying the seeds at 60°C inactivated the enzyme. Drying of the seed at this temperature may be useful as a pretreatment for extraction of oil from the seed. |
Databáze: | OpenAIRE |
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