Measurement of lipase activity in rubber (Hevea brasiliensis ) seed

Autor: Obioma U. Njoku, I. C. Ononogbu, F. U. Eneh
Rok vydání: 1996
Předmět:
Zdroj: Journal of the American Oil Chemists' Society. 73:1471-1473
ISSN: 0003-021X
DOI: 10.1007/bf02523512
Popis: Lipase activity in para rubber (Hevea brasiliensis) seed was measured by monitoring the release of free fatty acid by lipolysis of the endogenous lipid in a crushed sample of seed incubated at 37.5°C for 30 min. Free fatty acid was determined colorimetrically by a modified copper soap method. Fresh seeds showed the highest lipase activity. Drying the seeds at 60°C inactivated the enzyme. Drying of the seed at this temperature may be useful as a pretreatment for extraction of oil from the seed.
Databáze: OpenAIRE