Differentiating tomato leaf curl viruses possessing mono/bi partite genomes using replicase gene based PCR assay: implications for developing virus specific resistance
Autor: | Mohammad Faisal, Abdulrahman A. Alatar, Vipin Permar, Elangovan Mani, Shelly Praveen, Vilas Parkhi, Varsha Garg |
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Rok vydání: | 2015 |
Předmět: | |
Zdroj: | Journal of Plant Biochemistry and Biotechnology. 24:461-465 |
ISSN: | 0974-1275 0971-7811 |
Popis: | Tomato leaf curl disease (ToLCD) is associated with begomoviruses, with their generic names as Tomato leaf curl viruses (ToLCVs) and Tomato yellow leaf curl viruses (TYLCVs). ToLCVs exist both with mono/bipartite genomes, whereas TYLCVs carry monopartite genomes. For management of these viruses, three resistance loci Ty-1 to Ty-3 are being deployed, which provides different degree of resistance. These viruses encode a replication initiator protein (Rep), indispensible for virus replication. The conserved/divergent sequences of rep gene(s) were used to develop mono/bipartite virus specific PCR assay. The implications of distinguishing viruses with mono/bipartite genomes for using virus specific resistance loci through breeding have been discussed. |
Databáze: | OpenAIRE |
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