New Sandwich-Type Enzyme-Linked Immunosorbent Assay for Human MxA Protein in a Whole Blood Using Monoclonal Antibodies Against GTP-Binding Domain for Recognition of Viral Infection
| Autor: | Akira Kusano, Akihito Tomita, Akiko Furuya, Hiroaki Kohno, Kyosuke Nagata, Yuichi Adachi, Toshiko Itazawa, Toshio Miyawaki, Nobuo Hanai, Akira Horiguchi, Mizuho Kawamura, Hideki Tanigaki, Yoshie Okabe |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
chemistry.chemical_classification GTP' medicine.drug_class Biochemistry (medical) Clinical Biochemistry Public Health Environmental and Occupational Health Hematology Biology Monoclonal antibody Virology Molecular biology Medical Laboratory Technology Enzyme chemistry medicine biology.protein Immunology and Allergy Hemoglobin Antibody Binding site Whole blood Binding domain |
| Zdroj: | Journal of Clinical Laboratory Analysis. 26:174-183 |
| ISSN: | 0887-8013 |
| Popis: | Objectives To develop a clinically significant and practical enzyme-linked immunosorbent assay (ELISA) for the detection of MxA protein in human whole blood, a biological marker of viral infection. Design and Methods A sandwich ELISA suitable for the measurement of human MxA protein in whole blood was developed using mouse monoclonal antibodies (mAbs) raised against the GTP-binding domain of human MxA protein. Prior to the assay, the whole blood sample was treated with special buffer to extract the MxA protein, improve its stability, and avoid interference from hemoglobin. Results This ELISA meets all the requirements for use in routine clinical assays, especially in terms of sensitivity (detection limit: 1.3 ng/ml whole blood), accuracy (recovery: 93.0–100.0%), and rapidity ( |
| Databáze: | OpenAIRE |
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