| Autor: | M Michael, L E Iype, P T Iype, M Verma |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Clinical Biochemistry
Biomedical Engineering Cancer Bioengineering Cell Biology Biology medicine.disease_cause medicine.disease Metastatic breast cancer Breast cancer Cell culture Immunology medicine Cancer research Cytotoxic T cell skin and connective tissue diseases Receptor Carcinogenesis Immortalised cell line Biotechnology |
| Zdroj: | Cytotechnology. 26:207-218 |
| ISSN: | 0920-9069 |
| DOI: | 10.1023/a:1007953429252 |
| Popis: | New human breast cell lines were developed from metastatic breast cancer tissues and normal breast tissues. Primary cultures were initiated from cellular outgrowths of explanted tissues or from mechanically isolated cells in two serum-free media. Cell cultures derived from both cancer and normal tissues were immortalized with pRSV-T plasmid to generate permanent breast cell lines that exhibited an epithelial morphology. Cell lines generated in this study were characterized with respect to morphology, growth rate, karyotype, presence of specific genes, and the expression of epithelial and breast markers. The cell lines expressed the epithelial cell markers, cytokeratins 8 and 18, and retained the capacity to produce human milk fat globulin. They also express the BRCA-1, erbB2, and EGF receptor genes and possess the H-ras, K-ras, and p53 genes. Preliminary data showed that one of the new cancer cell lines was highly sensitive to the cytotoxic action of taxol. It is envisioned that the new breast cell lines will be useful as targets for identification of therapeutic agents against breast cancer and as models for carcinogenesis studies. |
| Databáze: | OpenAIRE |
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